Genotyping and primer details


Genotyping and primer details for 500F07 - At4g11370
Line ID 500F07
Locus At4g11370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6908083 of line 500F07 Chr4:6908083
   
Insertion allele amplicon CR3S - 30ww
T-DNA primer CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Locus specific primer 30ww: 5'-TATACTTATCTTCAGCTGCCGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6907637
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11