Sequences from confirmation amplicon


Confirmation sequences for line 511D03 and hit At5g44316 are shown.
Line ID 511D03
Locus At5g44316 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17854065
Primers used for PCR 10td - 8474, show primer details
   
Sequence Name 58-K116920-0022-511-D03C-10td
GenBank Accession LN509810 [GenBank]
Sequencing primer gene specific primer, 10td: 5'-AGTGATGATAACTACTACGCGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K116920-0022-511-D03C-10td
CGCCGAGGAAGGTAGTTCTCGTTCGAGTATTCAGAAGGATCGCACTCGCTATTTCTCACG
ACAAGAATCAGCTTCACGCCGCCGTCGTCGAGCTCTATTGCGCTGAAGGAGCGGAGATTA
AGTACTCCACGGTGCAGAATTGATATGCAGGGGATGAACAAGGTAAAGGTGGGATTTACA
ATTTCGTCACAAAGCGTGGTCTCTGCGCTGGTGATCGTTCCAAGATCTCATGGACTCAAG
TTGAGAAAGGGTTCGCGATAACTTGGAAGTATCCAAGCGTGGTTTTAGAAGGACATATAT
CCATCTATGTGATTCATTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAG
   
Sequence Name 14-K116919-0022-511-D03C-8409
GenBank Accession LN492679 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116919-0022-511-D03C-8409
AATCACATAGATGGATATATGTCCTTCTAAAACCACGCNTTGGATACTTCCAAGTTATCG
CGAACCCTTTCTCAACTTGAGTCCATGAGATCTTGGAACGATCACCAGCGCAGAGACCAC
GCTTTGTGACGAAATTGTAAATCCCACCTTTACCTTGTTCATCCCCTGCATATCAATTCT
GCACCGTCGAGTACTTAATCTCCGCTCCTTCAGCGCAATAGAGCTCAACAACAGCAGCGT
GAAGCTGATTCTTGTCATGAGAAATAGCAGTGCATCCTTCTGAATACTCAACAAAACTAC
CTTCCTCAGCAACAATCAAAGTTCTTTCAAACTGACCAGTGTTTTCAATAGAATTCATCC
GAAAATAACTCGAAAGAGGCATCGGACATCTAGTGTTTTTCGGTATATAACAGAACGATC
CATCGCTGAAAACCGCGGTATTCAAAGCCGCGTAGTA

Last Updated on 06.12.2018 16:11