Genotyping and primer details


Genotyping and primer details for 511D03 - At5g44316
Line ID 511D03
Locus At5g44316 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17854065 of line 511D03 Chr5:17854065
   
Insertion allele amplicon 8474 - 10td
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10td: 5'-AGTGATGATAACTACTACGCGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17853619
   
wt amplicon (over InsPos) 02ue - 10td
wt amplicon start Chr5:17853619
wt amplicon end Chr5:17854458
wt amplicon size 840 bp
Locus specific primer 10td: 5'-AGTGATGATAACTACTACGCGGCT-3'
2nd locus specific primer 02ue: 5'-AGGCAACTTGTTGAAAACATCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11