Sequences from confirmation amplicon


Confirmation sequences for line 513H06 and hit At1g12360 are shown.
Line ID 513H06
Locus At1g12360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4204120
Primers used for PCR 36bk - 8474, show primer details
   
Sequence Name 28-K107979-0022-513-H06C-36bk
GenBank Accession LN498878 [GenBank]
Sequencing primer gene specific primer, 36bk: 5'-ATAAATTCGGTGCTCAGAGAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K107979-0022-513-H06C-36bk
CATTCGTCAGACACTAAATCCTGTGAAATGTTAATCAAATGATGCATATTAATAGAGTAA
TTGTGAGTAGCACTACCAAAAGTTGAAGTGGAGGTGGACGCAACAAGAAAAGAAAAGGAG
AGAATTGTACAAGTATGGAACTACCTCTACGTGGAGAGAGAGCTTGTCAATTTGTTCACT
GTACTGTGGCAATGCTTGAACCATCTTCTGCAAATCTCTTGTAGAAAGCTCAGCTCCATC
TCTAAGAAAAAAGAGTCAAACAAAATTGATCAAAAGGAGAGAAGACCTACTTCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTA
   
Sequence Name 12-K107978-0022-513-H06C-8409
GenBank Accession LN491611 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K107978-0022-513-H06C-8409
TTTACATTGAATAATCCTGAAGTAGGTCTTCTCTCCTTTTGATCAATTTTGTTTGACTCT
TTTTTCTTAGAGATGGAGCTGAGCTTTCTACAAGAGATTTGCAGAAGATGGTTCAAGCAT
TGCCACAGTACAGTGAACAAATTGACAAGCTCTCTCTCCACGTAGAGGTAGTTCCATACT
TGTACAATTCTCTCCTTTTCTTTTCTTGTTGCGTCCACCTCCACTTCAACTTTTGGTAGT
GCTACTCACAATTACTCTATTAATATGCATCATTTGATTAACATTTCACAGGATTTAGTG
TCTGACGAATTGTCTGCACATGTCCAGAGTTATTGCTTCTCTGGCTTTTTCTCTGAGCAC
CGAATTTAT

Last Updated on 06.12.2018 16:11