Genotyping and primer details


Genotyping and primer details for 513H06 - At1g12360
Line ID 513H06
Locus At1g12360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4204120 of line 513H06 Chr1:4204120
   
Insertion allele amplicon 8474 - 36bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36bk: 5'-ATAAATTCGGTGCTCAGAGAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4204470
   
wt amplicon (over InsPos) 03cr - 36bk
wt amplicon start Chr1:4203650
wt amplicon end Chr1:4204470
wt amplicon size 821 bp
Locus specific primer 36bk: 5'-ATAAATTCGGTGCTCAGAGAAAAA-3'
2nd locus specific primer 03cr: 5'-TGGATACTCCATGTGATGTTCATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11