Sequences from confirmation amplicon


Confirmation sequences for line 567A04 and hit At4g34620 are shown.
Line ID 567A04
Locus At4g34620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16535771
Primers used for PCR CE53 - 8474, show primer details
   
Sequence Name 02-K105749w-0022-567-A04C-CE53
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, CE53: 5'-ACCCACCAGCTTAAATAAAAACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K105749w-0022-567-A04C-CE53
GACCTCAATTTCCAGCAGAGATGACGGTGAAGATAAGGCTCGCGCGCTCTTGGTTGTAAC
ACCGACCCTTCTATCGTGTAGTTGTCGCCGATGAAAAATCGCGCAGGGACGGTAAACAAA
TCGAGGTGTTAGGCTTTTATGATCCACTCCAAGGTTCGTTATTTCTCCTCTATATACATT
TCTCAATTCTCAGTAAAATTGAACACTTCCTAATGTCTCTGCTATCATGAATCTACTTGT
ACGTCTTCAGGCAAAGAAGATGCGGATAGAGTGAGCCTCAAATTCGACAGAATCAAGTAT
GTTTTTTTTACTTGTTCTATCATCTTCATATTCCTGTTTGCTCAATGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACG
ATTTAT
   
Sequence Name 58-K105750-0022-567-A04C-8409
GenBank Accession LN509709 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K105750-0022-567-A04C-8409
GAGCCTTTACAATTGAATATATCATTGAGCAAACAGGAATATGAAGATGATAGAACAAGT
AAAAAAAACATACTTGATTCTGTCGAATTTGAGGCTCACTCTATCCGCATCTTCTTTGCC
TGAAGACGTACAAGTAGATTCATGATAGCAGAGACATTAGGAAGTGTTCAATTTTACTGA
GAATTGAGAAATGTATATAGAGGAGAAATAACGAACCTTGGAGTGGATCATAAAAGCCTA
ACACCTCGATTTGTTTACCGTCCCTGCGCGATTTTTCATCGGCGACAACTACACGATAGA
AGGGTCGGTGTTTACAACCAAGACGCGCGAGCCTTATCTTCACCGTCATCTCTGCTTGGA
AATTGAGGTCTGAGAAGTGAGAAGAAAGAAGATAAGTAAAGGTTTTTATTTAGCTTGGTG
GGT
Primers used for PCR CE54 - CR3S, show primer details
   
Sequence Name 55-K118077-0022-567-A04C-CE54
GenBank Accession LN508934 [GenBank]
Sequencing primer gene specific primer, CE54: 5'-GTTAGACACCAAGAACTGTGTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118077-0022-567-A04C-CE54
TCAATCATCATACAAGCAAAACCAAGCCTCACAGAAAACTTGCACCAGATGAAATGCATT
AGGTGTAGAAACAACCAAACATGACTGAGAGAAGCAAATGAATTAAGGATTGTAGCCTTA
TGCAAATAGAGTTTTGGACACAGAATCAACAGAAAGCATCTCAATTTGTCACAATCTAAG
CAATTAGGCAGCATCATTGTGGCAAGAACAAGTTTATGACCTTGCAGAATACTAAAGAGA
ATGAATTAAGATGATTAAAAGACAAACAAGAAAACACATTCATGAGGAATAATCAATCAT
ATGGATAATGTCGAGAAAGAGACTCTTTAGTGTTTCAGTCTTATCAAACAATCATGAGTT
TCACATCACCTGGATTTTGGTTTTAGGAATTAGAAATTTTATTGATAGAAGTATTTTACA
AATACAAATACATACTAAGGGTTTCTTATAATGCTCAA
   
Sequence Name 30-K118076-0022-567-A04C-CR3S
GenBank Accession LN499906 [GenBank]
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118076-0022-567-A04C-CR3S
CATAAATTTCTAATTCCTAAAACAAAATCCAGGTGATGTGAAACTCATGATTGTTTGATA
AGACTGAAACACTAAAGAGTCTCTTTCTCGACATTATCCATATGATTGATTATTCCTCAT
GAATGTGTTTTCTTGTTTGTCTTTTAATCATCTTAATTCATTCTCTTTAGTATTCTGCAA
GGTCATAAACTTGTTCTTGCCACAATGATGCTGCCTAATTGCTTAGATTGTGACAAATTG
AGATGCTTTCTGTTGATTCTGTGTCCAAAACTCTATTTGCATAAGGCTACAATCCTTAAT
TCATTTGCTTCTCTCAGTCATGTTTGGTTGTTTCTACACCTAATGCATTTCATCTGGTGC
AAGTTTTCTGTGAGGCTTGGTTTTGCTTGTATGATTGATTTGATTAGACTGGTTTAAAAG
TCTAAATCCACACAGTT

Last Updated on 06.12.2018 16:11