Genotyping and primer details


Genotyping and primer details for 567A04 - At4g34620
Line ID 567A04
Locus At4g34620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16535771 of line 567A04 Chr4:16535771
   
Insertion allele amplicon 8474 - CE53
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CE53: 5'-ACCCACCAGCTTAAATAAAAACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16536166
   
Insertion allele amplicon CR3S - CE54
T-DNA primer CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Locus specific primer CE54: 5'-GTTAGACACCAAGAACTGTGTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16535319
   
wt amplicon (over InsPos) CE54 - CE53
wt amplicon start Chr4:16535319
wt amplicon end Chr4:16536166
wt amplicon size 848 bp
Locus specific primer CE53: 5'-ACCCACCAGCTTAAATAAAAACCT-3'
2nd locus specific primer CE54: 5'-GTTAGACACCAAGAACTGTGTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11