Sequences from confirmation amplicon


Confirmation sequences for line 584A07 and hit At4g00220 are shown.
Line ID 584A07
Locus At4g00220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:90967
Primers used for PCR 25ve - 8474, show primer details
   
Sequence Name 63-K117894-0022-584-A07C-25ve
GenBank Accession LN511077 [GenBank]
Sequencing primer gene specific primer, 25ve: 5'-TATTTATGATGGGAAATTGCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K117894-0022-584-A07C-25ve
TTTATTTAATCATTTTCTATGTAATTATGTTTTAACTTCTTCTTTTTTTTTGAAGAAAAT
TGCGTTTTCAAACTTTTTTTTTAAAAGGAAATTTTCCATTTGGCCTAAGGGTCCAATAAA
CCACCAAAGTAAAGACCTTTTCCCCATCCAAGGGACCCCCCCGGATGGCCATTCCAGGGA
CCAAACCCCACCAGGCAAGGAGGCAAATACCCCTTCCTAAGGCACCAATTTTTTTTTTAA
ATCCACCCCAATTGGTCCTTTAAGGGAAAAAAATTTTTGGTTTTGCCCAGGACCAAAACC
CACCCCCCCTTAAAAAAAAAATTTTAAAAACCCAAAATTTTAACACCGGGGTCCGGGGCC
TTGCCCTTTGGGGAAAAATAAGGCCTTAAAAAAAAAGGCCGGGAAAAGGGCTCCAGGCCG
GGAAAACCCCCGGGGACCCCCAGGGATAAGAAGGGCCAATGGGGAAAAAAAAAAAAAGAA
TTCCCCATTTTTTTTCCATTCAAAAAGGGAAAATACCCCCAAATTTTAAA
   
Sequence Name 52-K117893-0022-584-A07C-8409
GenBank Accession LN507944 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K117893-0022-584-A07C-8409
GACATTTAATATAAGACATAACTTTGCCCAAAAGGCAAAGGCCATGAACCCAGTTGTAAA
ATTTTAGATTTTTAAAACTCTTATTTTAATGTAGAGTTGGTTTTCGATCATGGCAAAAAC
AAAAAATATTCATCCTTAAAAGAACAAATCGGAATGAATTAAAAAAAAAAATCGATGCAT
AAGAAAGGGTAATTTGCATCATTGCATGATGGGATATGATCCTAGAAATGACAATCAGAG
GAGATCCTTAAAATGGGAAAAAGTACTTAACTTTGTAGTTTAATTGAACATTAAGTCAAA
ATGAAAAATCTCATTTAAAAAAAAAAGTTTGGAAACCCCATTTTTTTTCAAAAAAAAAAA
AAAGTTTAAAACCTTTTTTCCCAAAAAAATGTTAAAATTAAAAGGTCCCGGTTGCCATTT
TAAAGCCGGAAAACCTTTTAAGAGTTTTTCCTTTTCCCAAGAAAAAAAAAGGGAAAAAAG
GGGCCGTTTTCCCTTTTTTTTTAAATTTAAAA

Last Updated on 06.12.2018 16:11