Genotyping and primer details


Genotyping and primer details for 584A07 - At4g00220
Line ID 584A07
Locus At4g00220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:90967 of line 584A07 Chr4:90967
   
Insertion allele amplicon 8474 - 25ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25ve: 5'-TATTTATGATGGGAAATTGCAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:90498
   
wt amplicon (over InsPos) 39vw - 25ve
wt amplicon start Chr4:90498
wt amplicon end Chr4:91689
wt amplicon size 1192 bp
Locus specific primer 25ve: 5'-TATTTATGATGGGAAATTGCAACA-3'
2nd locus specific primer 39vw: 5'-TTGAAATGGATAATAATGCCAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11