Sequences from confirmation amplicon


Confirmation sequences for line 600G07 and hit At1g73980 are shown.
Line ID 600G07
Locus At1g73980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27820710
Primers used for PCR 22eq - 8474, show primer details
   
Sequence Name 22-K110827-0022-600-G07C-22eq
GenBank Accession LN496164 [GenBank]
Sequencing primer gene specific primer, 22eq: 5'-TCTCTCTATGCGAGTCTTTTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K110827-0022-600-G07C-22eq
TTGATGCTTATCTGACGCTGTCCACGAGCAACCAGTACGACCTGTCCAGATGATAAAAAT
ATTATAAAGTCACGAAGAGAAATGATTTATATTTCAAATCGTGAGCCTAGATCTCTATCT
TATATTCATACTTCTATCGAGTCTAAAGCTCTCTTTGATGCATCTGATGCTCTGTTGTTG
TCTATTGGAGTGAAGCAATCAATAATAGAACTGAGCATATGTGATTTTGATTTTGGAGAG
TTGGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 06-K110826-0022-600-G07C-8409
GenBank Accession LN488867 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K110826-0022-600-G07C-8409
GCCATTTACATTGAATATATCCTGACCACTCTCCAAATCAAAATCACATATGCTCAGTTC
TATTATTGATTGCTTCACTCCAATAGACAACAACAGAGCATCAGATGCATCAAAGAGAGC
TTTAGACTCGATAGAAGTATGAATATAAGATAGAGATCTAGGCTCACGATTTGAAATATA
AATCATTTCTCTTCGTGACTTTATAATATTTTTATCATCTGGACAGGTCGTACTGGTTGC
TCGTGGACAGCGTCAGATAATGCATCAAATGGACACACTGAGTAATCTTCTTCGGGAGTA
TGTTGGAGAAAAGACTCGCATAGAGAGA

Last Updated on 06.12.2018 16:11