Genotyping and primer details


Genotyping and primer details for 600G07 - At1g73980
Line ID 600G07
Locus At1g73980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27820710 of line 600G07 Chr1:27820710
   
Insertion allele amplicon 8474 - 22eq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22eq: 5'-TCTCTCTATGCGAGTCTTTTCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27820409
   
wt amplicon (over InsPos) 08fl - 22eq
wt amplicon start Chr1:27820409
wt amplicon end Chr1:27821311
wt amplicon size 903 bp
Locus specific primer 22eq: 5'-TCTCTCTATGCGAGTCTTTTCTCC-3'
2nd locus specific primer 08fl: 5'-TGAAATATCTTCACGGGTATCCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11