Sequences from confirmation amplicon


Confirmation sequences for line 605B02 and hit At5g44260 are shown.
Line ID 605B02
Locus At5g44260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17830168
Primers used for PCR 26us - 8474, show primer details
   
Sequence Name 85-K117671-0022-605-B02C-26us
GenBank Accession LN514585 [GenBank]
Sequencing primer gene specific primer, 26us: 5'-TAATAAACAATGTACCACGACGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K117671-0022-605-B02C-26us
GGAAAGATCTCAGNTAGTTCACAGTTGATGTCATACTGTTCATGTCAAAAGATCGTTCAC
CCATCCTAGATCAAGATCGCCACTAGAACCGACTTCAAAGCTATTTTTATCCCTAGCTTG
GGCCGCCGCAGCTGCAGCCACCGAGAAATCGCTTAGCCGGCCACCACGTGGTGTGAATTC
ATCGCCAAAGAAGCTCGACGGTGGTGGCTGAAGCTGGCCATGGAGGTAACTTGGCCGAAA
TCAATTGTAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 37-K117670-0022-605-B02C-8409
GenBank Accession LN502766 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117670-0022-605-B02C-8409
CATTTACAATTGGTAATTACAATTGATTTCGGCCAAGTTACCTCCATGGCCAGCTTCAGC
CACCACCGTCGAGCTTCTTTGGCGATGAATTCACACCACGTGGTGGCCGGCTAAGCGATT
TCTCGGTGGCTGCAGCTGCGGCGGCCCAAGCTAGGGATAAAAATAGCTTTGAAGTCGGTT
CTAGTGGCGATCTTGATCTAGGATGGGTGAACGATCTTTTGACATGAACAGTATGACATC
AACTGTGAACTACTTGAGATCTTTCCCGACGGGAATAATTATACGGCGGCGCCGCGGCGG
AGGTCCGTCGTGGTACATTGTTTATTA

Last Updated on 06.12.2018 16:11