Genotyping and primer details


Genotyping and primer details for 605B02 - At5g44260
Line ID 605B02
Locus At5g44260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17830168 of line 605B02 Chr5:17830168
   
Insertion allele amplicon 8474 - 26us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26us: 5'-TAATAAACAATGTACCACGACGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17829874
   
wt amplicon (over InsPos) 15vl - 26us
wt amplicon start Chr5:17829874
wt amplicon end Chr5:17830541
wt amplicon size 668 bp
Locus specific primer 26us: 5'-TAATAAACAATGTACCACGACGGA-3'
2nd locus specific primer 15vl: 5'-TAATCGATGTTGCTTGTTTTGTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11