Sequences from confirmation amplicon


Confirmation sequences for line 635C09 and hit At2g01580 are shown.
Line ID 635C09
Locus At2g01580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:265408
Primers used for PCR 19tg - 8474, show primer details
   
Sequence Name 33-K116676-0022-635-C09C-19tg
GenBank Accession LN501148 [GenBank]
Sequencing primer gene specific primer, 19tg: 5'-TTTTCCAAAAGGCTAATCGTTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116676-0022-635-C09C-19tg
TCCAATCATATTCAAAGGGAATCATAAGAACACAACAGAAGATTATGATCCACCATTAGA
AAGAAGCTTTTAGCTATCCCAAAAAAGAAAGAAAGAGAGATCCATTCATTGGTAATGGCA
GATTCCGACATAGAGACGAGCAGCAACCAAAATAGCAATAGCAACACCAGCCATTCAACT
ACTTCAACCACGAGTGTTCATGTTACGGCTCTCGATGGGATTGTAAGTGCTAATTCTCTC
TTCACGGTTGCAGTCTTTGTCGGAATCTCCTTCGACCAGCCAAGTGACCTCACCCTCACG
GACAGAACAGAGTGCAGTGCAGGACGAGACGTGGAACGTGACCTGGTTGTGTTTGAAGTC
ATCTCATTTGCCTTCTTTCTCTTCTCCTCCCTTGTGGCTCAAGGGATGAAGCTAGCCATC
AACCTTTTGAACAGCAAAGAAACGGACGAAGTGTTTAAAGCGAATATCAACAGAGATGTG
CTTCGTTTCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 25-K116675-0022-635-C09C-8409
GenBank Accession LN497619 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K116675-0022-635-C09C-8409
TCGCTTTAACACTTCGTCCGNTTTCTTTGCTGTTCAAAGGTTGATGGCTAGCTTCATCCC
TTGAGCCACAAGGGAGGAGAAGAGAAAGAAGGCAAATGAGATGACTTCAAACACAACCAG
GTCACGTTCCACGTCTCGTCCTGCACTGCACTCTGTTCTGTCCGTGAGGGTGAGGTCACT
TGGCTGGTCGAAGGAGATTCCGACAAAGACTGCAACCGTGAAGAGAGAATTAGCACTTAC
AATCCCATCGAGAGCCGTAACATGAACACTCGTGGTTGAAGTAGTTGAATGGCTGGTGTT
GCTATTGCTATTTTGGTTGCTGCTCGTCTCTATGTCGGAATCTGCCATTACCAATGAATG
GATCTCTCTTTCTTTCTTTTTTGGGATAGCTAAAAGCTTCTTTCTAATGGTGGATCATAA
TCTTCTGTTGTGTTCTTATGATTCCCTTTTGAATATGATTTGGTAATGTTGTTTAAGAGT
GGGTGGTGTTTGGTTTAGAACGATTAGCCTTTTGGAAAAATAAGTCCCTCAGCTTTTACC
ACTTACTGGACA

Last Updated on 06.12.2018 16:11