Genotyping and primer details

Genotyping and primer details for 635C09 - At2g01580
Line ID 635C09
Locus At2g01580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:265408 of line 635C09 Chr2:265408
Insertion allele amplicon 8474 - 19tg
Locus specific primer 19tg: 5'-TTTTCCAAAAGGCTAATCGTTCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:265927
wt amplicon (over InsPos) 02tu - 19tg
wt amplicon start Chr2:264899
wt amplicon end Chr2:265927
wt amplicon size 1029 bp
Locus specific primer 19tg: 5'-TTTTCCAAAAGGCTAATCGTTCTA-3'
2nd locus specific primer 02tu: 5'-AGAGAGCTTCAAGGAAACACTTTG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11