Sequences from confirmation amplicon


Confirmation sequences for line 658F06 and hit At2g36390 are shown.
Line ID 658F06
Locus At2g36390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15266746
Primers used for PCR 13ow - 8474, show primer details
   
Sequence Name 43-K115075-0022-658-F06C-13ow
GenBank Accession LN504943 [GenBank]
Sequencing primer gene specific primer, 13ow: 5'-AGACCAAACTTTCCACTATCCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115075-0022-658-F06C-13ow
TTTAAACTTTCTACCTTTGTCAGTGACATTATGAACTTAGTGATTACCATTCCAAGATAA
GAGAAAGAAACATACCGTACTACTCATTCCAACATGTGATTCATATATACGCAGCGATGT
GGGTTTCTTTGGACGAGGATGTTTGAACGCATATTTATCCTGAAACAATGGAGTACTTTT
TAGGCCTTCAGCGAGGGATATATATATATATATATATATGAAACCACATATACCCATAGA
TAATGCTGGATACCAGTTATGACTTAAATACTACATATTATGTTTTTATTGGATGTCTAC
CTCCTGAAAGAGTTTCGATTATCTCTAGAAAACACCAAAACAAATGAGAATGTAAAATGA
GTACTGGTTTGTACCTCCTCAAGAGGGTCATAATATACTCCATTATATGGGATCTCGCCG
GGTGGCTGGACAGAATACTTGATCCAAGCTGGAATGGAGTCTTTAATACCAGATGGGGTA
TCCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACGTGGATCAGCAATGAGT
ATGATGGTCTATATGGAGAAAAAGAAAGAGTAATTACCATTTTTTTTTCAATTCAAAAAT
GT
   
Sequence Name 11-K115074-0022-658-F06C-8409
GenBank Accession LN491240 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115074-0022-658-F06C-8409
TTTACATTGAATATTGGATACCCCATCTGGTATTAAAGACTCCATTCCAGCTTGGATCAA
GTATTCTGTCCAGCCACCTGGCGAGATCCCATATAATGGAGTATATTATGACCCTCCTGA
GGAGGTACAAACCAGTACTCATTTTACATTCTCATTTGTTTTGGTGTTTTCTAGAGATAA
TCGAAACTCTTTCAGGATGTAGACATCCAATAAAAACATAATATGTAGTATTTAAGTCAT
AACTGGTATCCAGCATTATCTATGGGTATATGTGGTTTCATATATATATATATATATATA
TCCCTCGCTGAAGGCCTAAAAAGTACTCCTTTGTTTCGAGATAAATATGCGTTCAAACAT
CCTCGTCCAAAGAAACCCACATCGCTGCGTATATATAAATCACATGTTGGAATGAGTAGT
ACGGTGTGTTTCTCTCTCTTATCTGGGAGTGGTAATCACTAAGTTCATAATGTCACTGAC
AAAGGTAGAAGTTTAAACTACATTTGACATGAAAATTCTCATATGTGCCTGACTTGTTTG
CTTGTTGAGGATAGTGGAAGTTTTTG

Last Updated on 06.12.2018 16:11