Genotyping and primer details


Genotyping and primer details for 658F06 - At2g36390
Line ID 658F06
Locus At2g36390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15266746 of line 658F06 Chr2:15266746
   
Insertion allele amplicon 8474 - 13ow
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13ow: 5'-AGACCAAACTTTCCACTATCCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15267300
   
wt amplicon (over InsPos) 01ps - 13ow
wt amplicon start Chr2:15266338
wt amplicon end Chr2:15267300
wt amplicon size 963 bp
Locus specific primer 13ow: 5'-AGACCAAACTTTCCACTATCCTCA-3'
2nd locus specific primer 01ps: 5'-GGAGATTTTAATAACTGGAATGCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11