Sequences from confirmation amplicon


Confirmation sequences for line 676A06 and hit At5g59710 are shown.
Line ID 676A06
Locus At5g59710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24059012
Primers used for PCR HP47 - 8474, show primer details
   
Sequence Name 76-K033554-022-676-A06C-8409
GenBank Accession LN513331 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K033554-022-676-A06C-8409
ATTTACAATTGTAAATCAACATAATCTTACTCTGTCTTCACGACTAAATTTGGCAGCAAA
TAGCGGATCAGGATTAAATGTTCAGGGACAGAACCGAATGATGGGTGGAGTACTTCCTCA
AGGTATATATGATTTATCGAGTCCTTTCCATTTGCTGTCTTCAAGGTTTTTTTTGCCTTT
CATTTGATACAACTGAGCCGAGCCTTGTTTAACTTCTTGTTATAGCATTGTAAATAAAAT
AGAATATATTGCTTCATGGAGTACACCTTTTTACTGGTCTAGTAGCTAGGAGGTCCTGTG
GAAAATAAGTTAGAATAATTAGTCATTTAGGGGGTAGGATTGATATCAGCAGTTGAACTT
GTTTTGCTTTTGTAGTCTATACTTCTCTAGACTCACGTGAGTACGTTAGAATGTAGAGAA
AGCAGTACTCTGGGATTGTTTGGACTTTGTAAGACGAGCTTGTTTACTTTACACAAAATC
TTATTTAATTAAACATGCAATTAGTTAGCAGCTGCTGTATGTGGAAATAGCAGTAAAAAT
TTAATTGTAAAATTGTATGGTACAGGATCTCAGGTCATGTCTATGCTGGGG
   
Sequence Name 76-K033554-022-676-A06C-HP47
GenBank Accession LN513332 [GenBank]
Sequencing primer gene specific primer, HP47: 5'-TATGATAGGAGTTCCCCAGCATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K033554-022-676-A06C-HP47
CATTTTACATTAAATTTTTACTGCTATTTCCCATACAGCAGCTGCTAACTAATTGCATGT
TTAATTAAATAAGATTTTGTGTAAAGTAAACAAGCTCGTCTTACAAAGTCCAAACAATCC
CAGAGTACTGCTTTCTCTACATTCTAACGTACTCACGTGAGTCTAGAGAAGTATAGACTA
CAAAAGCAAAACAAGTTCAACTGCTGATATCAATCCTACCCCCTAAATGACTAATTATTC
TAACTTATTTTCCACAGGACCTCCTAGCTACTAGACCAGTAAAAAGGTGTACTCCATGAA
GCAATATATTCTATTTTATTTACAATGCTATAACAAGAAGTTAAACAAGGCTCGGCTCAG
TTGTATCAAATGAAAGGCAAAAAAAACCTTGAAGACAGCAAATGGAAAGGACTCGATAAA
TCATATATACCTTGAGGAAGTACTCCACCCATCATTCGGTTCTGTCCCTGAACATTTAAT
CCTGATCCGCTATTTGCTGCCAAATTTAGTCGTGAAGACAGAGTAAGATTATGTTGATTT
ACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTCAC

Last Updated on 06.12.2018 16:11