Genotyping and primer details


Genotyping and primer details for 676A06 - At5g59710
Line ID 676A06
Locus At5g59710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24059012 of line 676A06 Chr5:24059012
   
Insertion allele amplicon 8474 - HP47
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP47: 5'-TATGATAGGAGTTCCCCAGCATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24059585
   
wt amplicon (over InsPos) 07qy - HP47
wt amplicon start Chr5:24058636
wt amplicon end Chr5:24059585
wt amplicon size 950 bp
Locus specific primer HP47: 5'-TATGATAGGAGTTCCCCAGCATAG-3'
2nd locus specific primer 07qy: 5'-TTAATCTCTCTCAGGTGCATGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11