Sequences from confirmation amplicon


Confirmation sequences for line 687G06 and hit At1g04230 are shown.
Line ID 687G06
Locus At1g04230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1127487
Primers used for PCR 12ih - 8474, show primer details
   
Sequence Name 39-K112810-0022-687-G06C-12ih
GenBank Accession LN503409 [GenBank]
Sequencing primer gene specific primer, 12ih: 5'-AGTCAACGAGGCTAGTGGTGTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K112810-0022-687-G06C-12ih
GAGTCGATTGTGACTCTTTTCTGGCTAACGTTTCCGTAAAGATCATCTCTGACTGTTGTC
AGCGTTTTTTTCTCAGAGAGCCGGTTTCTAGTGCTTCTGGTAGAGCAACATCGAGCATGT
CCAGTGACGAAAGAAATCAGGTATGATGCGAAAGCTACTCACAGTTTTGTTGTTGCATGA
TACTGGGGACTCTTATACGCCGAGAAAGTCAGAAACGTAACTTGTTCAATTCCGGGGGGG
ATCAAAGAATATGAAAATGAAGTGTTATATATTGAATGTTAGTATTTTGGTAAGTGATTT
CTTTTCTTTTATTGTAGAAGCCATACTCTACTAGGGTTTTAATGCCACCACCACGGTCAA
GGTTTGCATCAGTATTCATCGAAACACTTCACATAAAAAATCAGGATATATCATAATTGA
TACCTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTGTTTTTCAATTCAAAAATGT
AGATGTCCGACACGGTTAT
   
Sequence Name 14-K112809-0022-687-G06C-8409
GenBank Accession LN492581 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K112809-0022-687-G06C-8409
ATTTACATTGAAGGTATCAATTATGATATATCCTGATTTTTTATGTGAAGTGTTTCGATG
AATACTGATGCAAACCTTGACCGTGGTGGTGGCATTAAAACCCTAGTAGAGTATGGCTTC
TACAATAAAAGAAAAGAAATCACTTACCAAAATACTAACATTCAATATATAACACTTCAT
TTTCATATTCTTTGATCCCCCCCGGAATTGAACAAGTTACGTTTCTGACTTTCTCGGCGT
ATAAGAGTCCCCAGTATCATGCAACAACAAAACTGTGAGTAGCTTTCGCATCATACCTGA
TTTCTTTCGTCACTGGACATGCTCGATGTTGCTCTACCAGAAGCACTAGAAACCGGCTCT
CTGAGAAAAAAACGCTGACAACAGTCAGAGATGATCTTTACTGGAAACGTTAAGCCAGAA
AAGAGTCAACAATCGACTTCTTCTAGCATAGAAATCATATGGAATTTAGAGGTGAATACA
CCACTAGCCTCGTTGAC

Last Updated on 06.12.2018 16:11