Genotyping and primer details


Genotyping and primer details for 687G06 - At1g04230
Line ID 687G06
Locus At1g04230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1127487 of line 687G06 Chr1:1127487
   
Insertion allele amplicon 8474 - 12ih
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12ih: 5'-AGTCAACGAGGCTAGTGGTGTATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1127055
   
wt amplicon (over InsPos) 15ji - 12ih
wt amplicon start Chr1:1127055
wt amplicon end Chr1:1127869
wt amplicon size 815 bp
Locus specific primer 12ih: 5'-AGTCAACGAGGCTAGTGGTGTATT-3'
2nd locus specific primer 15ji: 5'-GAGCTTCTTCGTTTTCTCAGACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11