Sequences from confirmation amplicon


Confirmation sequences for line 691G02 and hit At2g09970 are shown.
Line ID 691G02
Locus At2g09970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:3780204
Primers used for PCR NG77 - 8474, show primer details
   
Sequence Name 42-K102366-0022-691-G02C-8409
GenBank Accession LN504415 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102366-0022-691-G02C-8409
TTTACATTGAATATATCCACTTAGACAAACATTAGAAACACAATCATGATGATTATCTTG
ATCGTCTTTAGATGGACTATTAGGCATAGATCTAAGCATTCTTGGTGAATCTAAGCTTTT
TCTCAAGATCTGTCTCATCGCAGAGATCAAATGTCTTGTCAGATTCGATGGTGGGCTTGA
GGATTTGAACTTATTACACTTGTTCATGTGTCTCGCCATAGCTTCTTCTTCTGTTGTTAA
AAATCTTTTTGTTCTCATGACTTTGTACTTCACAGCTTCAGAACAAAATCCAGAGATCCA
TTTCCCTATGTAACGCTCTCTTATCATCTCTGTGTACGATTCTGTACACTCGTCGGTTAA
TCCGCAACAGTCACACGTCACCGAATCTAGCTCATCGCTGGATATTTGTGTCACCGGAAC
CGTTGAGATAATTATTACATCCGGTGTTGAAATTAAC
   
Sequence Name 42-K102367-0022-691-G02C-NG77
GenBank Accession LN504416 [GenBank]
Sequencing primer gene specific primer, NG77: 5'-TGTTAATTTCAACACCGGATGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102367-0022-691-G02C-NG77
ATATCCGCGATGAGCTAGATTCGGTGACGTGTGACTGTTGCGGATTAACCGACGAGTGTA
CAGAATCGTACACAGAGATGATAAGAGAGCGTTACATAGGGAAATGGATCTCTGGATTTT
GTTCTGAAGCTGTGAAGTACAAAGTCATGAGAACAAAAAGATTTTTAACAACAGAAGAAG
AAGCTATGGCGAGACACATGAACAAGTGTAATAAGTTCAAATCCTCAAGCCCACCATCGA
ATCTGACAAGACATTTGATCTCTGCGATGAGACAGATCTTGAGAAAAAGCTTAGATTCAC
CAAGAATGCTTAGATCTATGCCTAATAGTCCATCTAAAGACGATCAAGATAATCATCATG
ATTGTGTTTCTAATGTTTTGTCTAAGTGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11