Genotyping and primer details


Genotyping and primer details for 691G02 - At2g09970
Line ID 691G02
Locus At2g09970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:3780204 of line 691G02 Chr2:3780204
   
Insertion allele amplicon 8474 - NG77
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NG77: 5'-TGTTAATTTCAACACCGGATGTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:3780641
   
wt amplicon (over InsPos) 10tf - NG77
wt amplicon start Chr2:3779929
wt amplicon end Chr2:3780641
wt amplicon size 713 bp
Locus specific primer NG77: 5'-TGTTAATTTCAACACCGGATGTAA-3'
2nd locus specific primer 10tf: 5'-TTTGTTTAACTAAGCTCACAGCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11