Sequences from confirmation amplicon


Confirmation sequences for line 700B03 and hit At1g32530 are shown.
Line ID 700B03
Locus At1g32530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11762250
Primers used for PCR BC31 - 8474, show primer details
   
Sequence Name 45-K104023-0022-700-B03C-BC31
GenBank Accession LN505511 [GenBank]
Sequencing primer gene specific primer, BC31: 5'-CTAGAGTACCACCCGTACCAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K104023-0022-700-B03C-BC31
CAACTAGACCTATTCGTTGGGACATCCAGAGTACTTGCCCTACCAACATGGAGCTCACTC
ATTAACAAACACCACATTGCATCACCTTTACTCAAATTAGGCTTAACTTGTTGCAACAAG
TAAATCATACCCGCAAGCGAATACTCCTCCAAATCTCTCAAATCCGTAAAACCAGTCTCC
GTTCGATCCTCACCGTTCCCATTACTCCCACCTCCACCTCCACCACTATTCAAATAAGAT
AATGAATTATTCACTATATTAGTCAACACATCCNATTCACCATAACAGTGTCCATTACTC
AAAACCGCTTTCAACGCAACACGTTCCTCATAGCCCAATTCAAGAAGCTTAGAAACAGCT
TGATTATACAAAAACTCCAAATGTTTCAGCAAAATCTCTTCCAAATGCTCCTCTGTACAA
TATGCCCAAAAATTCTCCTCAGGATTCTCAGTAACGGAATTCAAATTGACAGAACCGGAA
TCAGTAAGACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCANAAATGTAGATNTCCG
   
Sequence Name 61-K104024-0022-700-B03C-8409
GenBank Accession LN510506 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K104024-0022-700-B03C-8409
TCGGACTGAGCCTTTACATTGAAATATCCTGGTCTTACTGATTCCGGTTCTGTCAATTTG
AATTCCGTTACTGAGAATCCTGAGGAGAATTTTTGGGCATATTGTACAGAGGAGCATTTG
GAAGAGATTTTGCTGAAACATTTGGAGTTTTTGTATAATCAAGCTGTTTCTAAGCTTCTT
GAATTGGGCTATGAGGAACGTGTTGCGTTGAAAGCGGTTTTGAGTAATGGACACTGTTAT
GGTGAATTGGATGTGTTGACTAATATAGTGAATAATTCATTATCTTATTTGAATAGTGGT
GGAGGTGGAGGTGGGAGTAATGGGAACGGTGAGGATCGAACGGAGACTGGTTTTACGGAT
TTGAGAGATTTGGAGGAGTATTCGCTTGCGGGTATGATTTACTTGTTGCAACAAGTTAAG
CCTAATTTGAGTAAAGGTGATGCAATGTGGTGTTTGTTAATGAGTGAGCTCCATGTTGGT
AGGGCAAGTACTCTGGATGTCCCAACGAANAGGTCTAGTTGTTGTACTAAGGAAGATAGC
AATGTANAAGATGTTGGTACGGGTGGTACTCTA

Last Updated on 06.12.2018 16:11