Sequences from confirmation amplicon


Confirmation sequences for line 706H05 and hit At2g25850 are shown.
Line ID 706H05
Locus At2g25850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11030163
Primers used for PCR NH85 - 8474, show primer details
   
Sequence Name 55-K102594-0022-706-H05C-8409
GenBank Accession LN508774 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K102594-0022-706-H05C-8409
GTTTCTTCCTTGCTCTCGTAGAGCCCCTCATCAACCAGAAACTTCACAATCAATCAGCCA
AAACGCAAGTCAATATCACATTGTTCCTGAAAGCAATTTTTCATTGGAACAAAAAAACAA
ACAAACACAACGCTAACCTTCTCTAGTTCCAAATTACGCTTAACATCAGCAGCAGAAGGT
CCAGCAATAGACAGTGGCTCCGTGATCCCATAGCTCTTAAGAGAAGCTTTTACCGGTTGA
GAAGAGTCATCGTCCGTGCGTTGTTGAGTACTCACCATGACAAGACGCGTAAACACTAGA
AAATCCTAGGCGATTCAAGCACAAAATATAGATATCTCGATCTGATTAAGCACCCAGATC
ATGATAATTCGGAATCTAACAGGAATTGGAGACTTTAGAGTCATGGGAGAGAGATTGAAG
AAGGAGAAAAGAGCCAAAGGAGGAAAGAAAAAGTGAGAGAAGAGCGAGAAAAGCTCGAAA
ACGAAATCGGAAACCCTAAATCGATGGATAGGGAAAGCTCTCGACTGGAATTTTCAAAGG
GAAGTCAAAG
   
Sequence Name 55-K102595-0022-706-H05C-NH85
GenBank Accession LN508775 [GenBank]
Sequencing primer gene specific primer, NH85: 5'-TGATATTTTCCGCTTTCTTTGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K102595-0022-706-H05C-NH85
TTTCCTATCCTCGATTTAGGGTTTCCGATTTCGTTTTCGAGCTTTCTCTTGCTCTTCTCT
CACTTTTTCTTTCCTCCTTTGGCTCTTTTCTCCTTCTTCAATCTCTCTCCCATGACTCTA
AAGTCTCCAATTCCTGTTAGATTCCGAATTATCATGATCTGGGTGCTTAATCAGATCGAG
ATATCTATATTTTGTGCTTGAATCGCCTAGGATTTTCTAGTGTTTACGCGTCTTGTCATG
GTGAGTACTCAACAACGCACGGACGATGACTCTTCTCAACCGGTAAAAGCTTCTCTTAAG
AGCTATGGGATCACGGAGCCACTGTCTATTGCTGGACCTTCTGCTGCTGATGTTAAGCGT
AATTTGGAACTAGAGAAGGTTAGCGTTGTGTTTGTTTGTTTTTTTGTTCCAATGAAAAAT
TGCTTTCAGGAACAATGTGATATTGACTTGCGTTTTGGCTGATTTATTGTGAAGTTTCTG
GTTGATGAGGGGCTCTACGAGAGCAAGGAAGAAACTATGCGGAGAGAGGAAGTTGTGGTT
CGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11