Genotyping and primer details


Genotyping and primer details for 706H05 - At2g25850
Line ID 706H05
Locus At2g25850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11030163 of line 706H05 Chr2:11030163
   
Insertion allele amplicon 8474 - NH85
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NH85: 5'-TGATATTTTCCGCTTTCTTTGACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11030728
   
wt amplicon (over InsPos) 49rd - NH85
wt amplicon start Chr2:11029695
wt amplicon end Chr2:11030728
wt amplicon size 1034 bp
Locus specific primer NH85: 5'-TGATATTTTCCGCTTTCTTTGACT-3'
2nd locus specific primer 49rd: 5'-ATCTGATCAGTATAGCCCCTCTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11