Sequences from confirmation amplicon


Confirmation sequences for line 707G06 and hit At4g28320 are shown.
Line ID 707G06
Locus At4g28320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14019229
Primers used for PCR AP20 - 8474, show primer details
   
Sequence Name 51-K106187-0022-707-G06C-AP20
GenBank Accession LN507509 [GenBank]
Sequencing primer gene specific primer, AP20: 5'-ACCTGAAGAGCTCAATGCAATTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K106187-0022-707-G06C-AP20
GATCAGGGTAGATATGAACGGATGCAAATCTATGTTTGAGGAGTTAGAGTTTTGAACAAA
GTCTGTTCCGAGCTGGGATGCCCACTGTTCTGGATTAACTGTGAGCCCTTTCGGGCTATT
AGGACCATAGAAGCCTTCCAGACCTACTGTGAGGAGATGCTTGTCATCGATTGACTTAAT
GAATCCCGTCATTTCGTCTATCCAATCCTGTGATCCAGCAAAGAAATACATAAATATATT
TGCTGAATAAGAAACAATCTGAACGATTCCAAGTGGCAAGTGAAATGGAATACTCACTTG
GAGAGTTTTGCCAGAGACATCAGTCGTGCATCGAGGCTCGTTTATCAACTCCCAAGCGAA
AATCGTAGGATCGTTTCTGTACTCTATTCCCGTTACAGAGTTCTTCCTGGTGAGCAGAAC
CTGCGAATTGTAAATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 35-K106188-0022-707-G06C-8409
GenBank Accession LN501841 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K106188-0022-707-G06C-8409
ATTCGCAGGTTCTGCTCACCAGGAAGAACTCTGTAACGGGAATAGAGTACAGAAACGATC
CTACGATTTTCGCTTGGGAGTTGATAAACGAGCCTCGATGCACGACTGATGTCTCTGGCA
AAACTCTCCAAGTGAGTATTCCATTTCACTTGCCACTTGGAATCGTTCAGATTGTTTCTT
ATTCAGCAAATATATTTATGTATTTCTTTGCTGGATCACAGGATTGGATAGACGAAATGA
CGGGATTCATTAAGTCAATCGATGACAAGCATCTCCTCACAGTAGGTCTGGAAGGCTTCT
ATGGTCCTAATAGCCCGAAAGGGCTCACAGTTAATCCAGAACAGTGGGCATCCCAGCTCG
GAACAGACTTTGTTCAAAACTCTAACTCCTCAAACATAGATTTTGCATCCGTTCATATCT
ACCCTGATCACTGGTAAGTGAGCTGGATCAGAGAATCAAGATATAATTGCATTG
Primers used for PCR AP21 - 8474, show primer details
   
Sequence Name 52-K106187-0022-707-G06C-AP21
GenBank Accession LN507825 [GenBank]
Sequencing primer gene specific primer, AP21: 5'-ACCTGAAGAGCTCAATGCAATTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K106187-0022-707-G06C-AP21
GATCGGGTAGATATGAACGGATGCAAATCTATGTTTGAGGAGTTAGAGTTTTGAACAAAG
TCTGTTCCGAGCTGGGATGCCCACTGTTCTGGATTAACTGTGAGCCCTTTCGGGCTATTA
GGACCATAGAAGCCTTCCAGACCTACTGTGAGGAGATGCTTGTCATCGATTGACTTAATG
AATCCCGTCATTTCGTCTATCCAATCCTGTGATCCAGCAAAGAAATACATAAATATATTT
GCTGAATAAGAAACAATCTGAACGATTCCAAGTGGCAAGTGAAATGGAATACTCACTTGG
AGAGTTTTGCCAGAGACATCAGTCGTGCATCGAGGCTCGTTTATCAACTCCCAAGCGAAA
ATCGTAGGATCGTTTCTGTACTCTATTCCCGTTACAGAGTTCTTCCTGGTGAGCAGAACC
TGCGAATTGTAAATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 36-K106188-0022-707-G06C-OriL
GenBank Accession LN502232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K106188-0022-707-G06C-OriL
CATTCGCAGGTTCTGCTCACCAGGAAGAACTCTGTAACGGGAATAGAGTACAGAAACGAT
CCTACGATTTTCGCTTGGGAGTTGATAAACGAGCCTCGATGCACGACTGATGTCTCTGGC
AAAACTCTCCAAGTGAGTATTCCATTTCACTTGCCACTTGGAATCGTTCAGATTGTTTCT
TATTCAGCAAATATATTTATGTATTTCTTTGCTGGATCACAGGATTGGATAGACGAAATG
ACGGGATTCATTAAGTCAATCGATGACAAGCATCTCCTCACAGTAGGTCTGGAAGGCTTC
TATGGTCCTAATAGCCCGAAAGGGCTCACAGTTAATCCAGAACAGTGGGCATCCCAGCTC
GGAACAGACTTTGTTCAAAACTCTAACTCCTCAAACATAGATTTTGCATCCGTTCATATC
TACCCTGATCACTGGTAAGTGAGCTGGATCAGAGAATCAAGATATAATTGCATTGACTCC
CTTCAG

Last Updated on 06.12.2018 16:11