Genotyping and primer details


Genotyping and primer details for 707G06 - At4g28320
Line ID 707G06
Locus At4g28320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14019229 of line 707G06 Chr4:14019229
   
Insertion allele amplicon 8474 - AP20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP20: 5'-ACCTGAAGAGCTCAATGCAATTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14018744
   
Insertion allele amplicon 8474 - AP21
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP21: 5'-ACCTGAAGAGCTCAATGCAATTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14018744
   
wt amplicon (over InsPos) 14au - AP21
wt amplicon start Chr4:14018744
wt amplicon end Chr4:14019565
wt amplicon size 822 bp
Locus specific primer AP21: 5'-ACCTGAAGAGCTCAATGCAATTAT-3'
2nd locus specific primer 14au: 5'-CTCTCTCTGTCCTTGTTTAATGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11