Sequences from confirmation amplicon


Confirmation sequences for line 715E05 and hit At5g63780 are shown.
Line ID 715E05
Locus At5g63780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25525381
Primers used for PCR MC11 - 8474, show primer details
   
Sequence Name 25-K115573-0022-715-E05C-MC11
GenBank Accession LN497592 [GenBank]
Sequencing primer gene specific primer, MC11: 5'-TGTTCACAAGTTTTTGTGTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115573-0022-715-E05C-MC11
TGGATCTATCACTTGAGAGATTTACTTACAAATCTGAAGAAGATGACTATTGTTAAAGCA
CAGAGACCACCAAGAAGTATGTGAAGTCCGCTCCTAGCAGTTTTCTGTACAAATTCAAAA
CAACCGTTAACCAAATAAACGTTATCGATATACCAGTCAGAGAGGCTTAGATATACAAGG
TTAGATCGATGGCATACCTTTCCAACACGAGGGGCAATGAGCCAGACCAAAGTAACAGTG
AGTAAACCTGTAGCAAGTAGAATCCCAGTACCTTCCACAGCCCATTTAGTTGCACGGTTT
TCCATAGGTAAGATGTCGAAGTTCACAACACCTAAAGGTTGGTCAGGGATAGCCGCAACA
GAACTATTGTTGTTGTTGTTGTTGTTCAGGGAATGGGGACCAAACCATGACGTTATCTCA
CTAAGTCGTTGCCTTCGAATGACAGCAACTTCATCTGAATCAATGGTTGAATCTGTATTC
ACCGGTAATACAGGGATTTGATCTCCTCCATCAGCAGTTCTTTCTCTTATTGCTGTGTAA
TCTCTCAAGGCAATGACCACTTTGTTGAAATCAGCAGTCTTTATATTTTCTGCAGGATGT
CCACAGATTTCACACACGGTGGATCCATGGTTGAGAAACCACTTGAGCGCGCAAGCGTAG
TGTACCAAAGCAAGCTCGTTTTTGCAAGAACATCCAAGCTCAAGCAATGCATCCTGATGC
TGCTGGATTCCCATTTCTATATCANTTGTGCAAATGAAAACTTCCGCATCAGGACTTGTT
AATTCGATGAAGCCAGATTCCCTTCCATTGCTTTTGACAACAGAATTCTTCAGCTCAGAC
TCTGTAGCTCTGCTGACATCATCAGCGGTTCCATCTACATTGCTTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGTTCAATATGGAGAAAAAG
AAAGAGTATTTACCAATTTTTTTCAATT
   
Sequence Name 15-K115572-0022-715-E05C-8409
GenBank Accession LN493110 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115572-0022-715-E05C-8409
TTGAAGCAATGTAGATGGAACCGCTGATGATGTCAGCAAAGCTACAGAGTCTGAGCTGAA
GAATTCTGTTGTCAAAAGCAATGGAAGGGAATCTGGCTTCATCGAATTAACAAGTCCTGA
TGCGGAAGTTTTCATTTGCACAAATGATATAGAAATGGGAATCCAGCAGCATCAGGATGC
ATTGCTTGAGCTTGGATGTTCTTGCAAAAACGAGCTTGCTTTGGTACACTACGCTTGCGC
GCTCAAGTGGTTTCTCAACCATGGATCCACCGTGTGTGAAATCTGTGGACATCCTGCAGA
AAATATAAAGACTGCTGATTTCAACAAAGTGGTCATTGCCTTGAGAGATTACACAGCAAT
AAGAGAAAGAACTGCTGATGGAGGAGATCAAATCCCTGTATTACCGGTGAATACAGATTC
AACCATTGATTCAGATGAAGTTGCTGTCATTCGAAGGCAACGACTTAGTGAGATAACGTC
ATGGTTTGGTCCCCATTCCCTGAACAACAACAACAACAACAATAGTTCTGTTGCGGCTAT
CCCTGACCAACCTTTAGGTGTTGTGAACTTCGACATCTTACCTATGGAAAACCGTGCAAC
TAAATGGGCTGTGGAAGGTACTGGGATTCTACTTGCTACAGGTTTACTCACTGTTACTTT
GGTCTGGCTCATTGCCCCTCGTGTTGGAAAGGTATGCCATCGATCTAACCTTGTATATCT
NAGCCTCTCTGACTGGTATATCGATNACGTTTATTTGGTTAACGGTTGTTTTGAATTTGT
ACAGAANACTGCTAGGAGCGGACTTCACATACTTCTTGGTGGTCTCTGTGCTTTAACAAT
AGTCATCTTCTTCAGATTTGTAAGTAAATCTCTCAAGTGATAGATCCATTCTAACAGATN
AGTAGTTCCTACTTTTTACTAATCCAAACACACAAA

Last Updated on 06.12.2018 16:11