Genotyping and primer details


Genotyping and primer details for 715E05 - At5g63780
Line ID 715E05
Locus At5g63780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25525381 of line 715E05 Chr5:25525381
   
Insertion allele amplicon 8474 - MC11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MC11: 5'-TGTTCACAAGTTTTTGTGTGTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25526325
   
wt amplicon (over InsPos) 44si - MC11
wt amplicon start Chr5:25525024
wt amplicon end Chr5:25526325
wt amplicon size 1302 bp
Locus specific primer MC11: 5'-TGTTCACAAGTTTTTGTGTGTTTG-3'
2nd locus specific primer 44si: 5'-GGTCCTTTGTATTATTGGACCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11