Sequences from confirmation amplicon


Confirmation sequences for line 760A07 and hit At5g01500 are shown.
Line ID 760A07
Locus At5g01500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:199868
Primers used for PCR 37jn - 8474, show primer details
   
Sequence Name 51-K113214-0022-760-A07C-8409
GenBank Accession LN507561 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113214-0022-760-A07C-8409
ATTTACATTGAATATATACCTCAATGAAACCAATAGCCTTCTTAGCACTTTGTTGCCCAG
CTCGAACACCATGTGTCTATGAATTAATAAGACATCATTTAAGAATCAATGTACATTACT
ACAAGCCTGTAACAAGAACACAATCACAAATACTCCATAGATTCCCCTCAAGGTTCTTGG
CTTGAAATAATGAGATATAAATACATGATCAATTTTATCATAGACCATGTCATGGAAGAG
TCTCTTAGGAAAAGAAAAAAGAAGAGGAAAATCAACAATGTCCCACAAGCAACAACTAGC
CGAAACGACAGCGTATTCGTTGTGCTCACCAGTCACCAGTTTTATTTTTATTTTACTTTT
TAAAATGGAGAAAACACACGTGCCAGAAAACCTTAAAAGTTGTTGAGAAGGAGAGGAGAG
AGAGCCCTTACCTGCATTAGGAGCTTTATTCGGTCAA
   
Sequence Name 51-K113215-0022-760-A07C-37jn
GenBank Accession LN507562 [GenBank]
Sequencing primer gene specific primer, 37jn: 5'-CTTGACCGAATAAAGCTCCTAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113215-0022-760-A07C-37jn
ACACTTTTAGGTTTTCTGGCACGTGTGTTTTCTCCATTTTAAAAGTAAAATAAAAATAAA
ACTGGTGACTGGTGAGCACAACGAATACGCTGTCGTTTCGGCTAGTTGTTGCTTGTGGGA
CATTGTTGATTTTCCTCTTCTTTTTTCTTTTCCTAAGAGACTCTTCCATGACATGGTCTA
TGATAAAATTGATCATGTATTTATATCTCATTATTTCAAGCCAAGAACCTTGAGGGGAAT
CTATGGAGTATTTGTGATTGTGTTCTTGTTACAGGCTTGTAGTAATGTACATTGATTCTT
AAATGATGTCTTATTAATTCATAGACACATGGTGTTCGAGCTGGGCAACAAAGTGCTAAG
AAGGCTATTGGTTTCATTGAGGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT

Last Updated on 06.12.2018 16:11