Genotyping and primer details


Genotyping and primer details for 760A07 - At5g01500
Line ID 760A07
Locus At5g01500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:199868 of line 760A07 Chr5:199868
   
Insertion allele amplicon 8474 - 37jn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 37jn: 5'-CTTGACCGAATAAAGCTCCTAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:199422
   
wt amplicon (over InsPos) 16tf - 37jn
wt amplicon start Chr5:199422
wt amplicon end Chr5:200262
wt amplicon size 841 bp
Locus specific primer 37jn: 5'-CTTGACCGAATAAAGCTCCTAATG-3'
2nd locus specific primer 16tf: 5'-GGCAGAATGTACAGAACAAAGTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11