Sequences from confirmation amplicon


Confirmation sequences for line 782G06 and hit At1g18870 are shown.
Line ID 782G06
Locus At1g18870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6518973
Primers used for PCR 31ve - 8474, show primer details
   
Sequence Name 31-K117967-0022-782-G06C-31ve
GenBank Accession LN500331 [GenBank]
Sequencing primer gene specific primer, 31ve: 5'-ATTTTCTTTCACTGTCTTTTCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117967-0022-782-G06C-31ve
ACAAAATGCTTATACCGAGATACATATTCTTCTTCTTTTTCCAGAGTCATTTGATAGAGG
AATGTATGCTGGACCTATTGGGTTCTTTGGTGGTGGGGAGAGTGAATTTTCTGTAGGCAT
ACGATCTGCTTTAGTCGAAAAGGTGACAACTCCCCTTAGTGTAGCAAACGGGTCATTCAC
TGTTAAATACAGTGCATTTATCTTGGTCCTTTTGTGTCTCCCAGGGTCTTGGAGCATTGA
TCTATGCAGGAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGATTATTAT
   
Sequence Name 15-K117966-0022-782-G06C-8409
GenBank Accession LN493165 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117966-0022-782-G06C-8409
ATTTACAATTGAATATATCCTGTTCCTGCATAGATCAATGCTCCAAGACCCTGGGAGACA
CAAAAGGACCAAGATAAATGCACTGTATTTAACAGTGAATGACCCGTTTGCTACACTAAG
GGGAGTTGTCACCTTTTCGACTAAAGCAGATCGTATGCCTACAGAAAATTCACTCTCCCC
ACCACCAAAGAACCCAATAGGTCCAGCATACATTCCTCTATCAAATGACTCTGGAAAAAG
AAGAAGAATATGTATCTCGGTATAAGCATATATTAGTAGATAGCTACTTCTACAGAGAAA
TTCTTTTTTCCGAAAAGACATGAAAAGAAAAT

Last Updated on 06.12.2018 16:11