Genotyping and primer details


Genotyping and primer details for 782G06 - At1g18870
Line ID 782G06
Locus At1g18870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6518973 of line 782G06 Chr1:6518973
   
Insertion allele amplicon 8474 - 31ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31ve: 5'-ATTTTCTTTCACTGTCTTTTCGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6518659
   
wt amplicon (over InsPos) 39vx - 31ve
wt amplicon start Chr1:6518659
wt amplicon end Chr1:6519553
wt amplicon size 895 bp
Locus specific primer 31ve: 5'-ATTTTCTTTCACTGTCTTTTCGGA-3'
2nd locus specific primer 39vx: 5'-CGGTAATCTAATTACGTTCGTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11