Sequences from confirmation amplicon


Confirmation sequences for line 786C06 and hit At1g61210 are shown.
Line ID 786C06
Locus At1g61210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22566942
Primers used for PCR 09vc - 8474, show primer details
   
Sequence Name 61-K117671-0022-786-C06C-09vc
GenBank Accession LN510611 [GenBank]
Sequencing primer gene specific primer, 09vc: 5'-TTATGTGGTATGTGGTTTTCCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K117671-0022-786-C06C-09vc
TCAATTTCTCCGGATGGGCGAACCTTATTTTGTGGTTTGGATGACAGCTTAAAGGTTTTG
ATTTTCTTTGTGATGAGCTTTACCATATAAAGGGTTCTCCAGAGTCAAAAACATTGATAT
CATAAGCTGATGTTCATAATTCAGGTTTATTCATGGGAACCTGTGGTTTGCCATGATGGT
GTTGATATGGGATGGTCAACGCTTGGTGACTTATGCATCAGCGAAGGAAAGCTCCTGGGT
TGTTCATACTACCAAAATTCAGTAGGGATTTGGGTCTCAGATATATCAGTAGAGAGACCT
TCTTGGATTAATTACTCTTTCTTTTTGCGGAGAGACCTTTGTAATTACTACCAAAATTTC
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAACAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCACAAAGTTATTAT
   
Sequence Name 13-K117670-0022-786-C06C-8409
GenBank Accession LN492232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117670-0022-786-C06C-8409
CCTGGAAATTTTGGTAGTAATTACAAAGGTCTCTCCGCAAAAAGAAAGAGTAATTAATCC
AAGAAGGTCTCTCTACTGATATATCTGAGACCCAAATCCCTACTGAATTTTGGTAGTATG
AACAACCCAGGAGCTTTCCTTCGCTGATGCATAAGTCACCAAGCGTTGACCATCCCATAT
CAACACCATCATGGCAAACCACAGGTTCCCATGAATAAACCTGAATTATGAACATCAGCT
TATGATATCAATGTTTTTGACTCTGGAGAACCCTTTATATGGTAAAGCTCATCACAAAGA
AAATCAAAACCTTTAAGCTGTCATCCAAACCACAAAATAAGGTTCGCCCATCCGGATGAA
ATTTGATTGAACGAACTCCAGTAGCCTGTGAAAACAAGGAAAACCACATACCACATAAAG
G

Last Updated on 06.12.2018 16:11