Genotyping and primer details


Genotyping and primer details for 786C06 - At1g61210
Line ID 786C06
Locus At1g61210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22566942 of line 786C06 Chr1:22566942
   
Insertion allele amplicon 8474 - 09vc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09vc: 5'-TTATGTGGTATGTGGTTTTCCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22566598
   
wt amplicon (over InsPos) 24vl - 09vc
wt amplicon start Chr1:22566598
wt amplicon end Chr1:22567352
wt amplicon size 755 bp
Locus specific primer 09vc: 5'-TTATGTGGTATGTGGTTTTCCTTG-3'
2nd locus specific primer 24vl: 5'-GAGTAATAAGTAAGCTACCGCCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11