Sequences from confirmation amplicon


Confirmation sequences for line 787F01 and hit At3g06860 are shown.
Line ID 787F01
Locus At3g06860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2165100
Primers used for PCR 33ve - 8474, show primer details
   
Sequence Name 17-K117874-0022-787-F01C-33ve
GenBank Accession LN494044 [GenBank]
Sequencing primer gene specific primer, 33ve: 5'-GAGTGGTTTTACTCACTCCACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117874-0022-787-F01C-33ve
CACATATCATGCCGCTACTTGAAATAGTTCGGACCAATCATACCTCTGCCCAAGTAATTG
TTGACCTGTTAGATGTTGGGAAGAAGATTAAGAAAACACCAGTTGTGGTGGGAAACTGCA
CAGGGTTTGCAGTGAATAGGATGTTCTTCCCTTACACACAGGCAGCTATGTTCCTTGTTG
AGTGTGGAGCAGATCCATATCTAATCGACAGGGCAATCAGCAAGTTTGGAATGCCAATGG
GTCCCTTCAGGTATATCGCATTGTAAAAATCCATGTCATTCACACGCATCATCTGTTAAC
TATACTTTTGTTGCTTATATGAACGAGCAGACTGTGTGACCTGGTTGGATTTGGTGTGGC
GATTGCAACCGCAACGCAGTTTATCGAGAACTTCTCAGAACGGACTTACAAATCAATGAT
TATTCCACTTATGCAAGAGAGCTGGTATGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 05-K117873-0022-787-F01C-8409
GenBank Accession LN488539 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117873-0022-787-F01C-8409
ACATTGAATATATCATACCAGCTCTCTTGCATAAGTGGAATAATCATTGATTTGTAAGTC
CGTTCTGAGAAGTTCTCGATAAACTGCGTTGCGGTTGCAATCGCCACACCAAATCCAACC
AGGTCACACAGTCTGCTCGTTCATATAAGCAACAAAAGTATAGTTAACAGATGATGCGTG
TGAATGACATGGATTTTTACAATGCGATATACCTGAAGGGACCCATTGGCATTCCAAACT
TGCTGATTGCCCTGTCGATTAGATATGGATCTGCTCCACACTCAACAAGGAACATAGCTG
CCTGTGTGTAAGGGAAGAACATCCTATTCACTGCAAACCCTGTGCAGTTTCCCACCACAA
CTGGTGTTTTCTTAATCTTCTTCCCAACATCTAACAGGTCAACAATTACTTGGGCAGAGG
TATGATTGGTCCGAACTATTTCAAGTAGCGGCATGATATGTGCTGGACTGCATCAGAAAA
ATGAAGGTACATGATTATGTGTGGAGTGATAAAAACCACTC

Last Updated on 06.12.2018 16:11