Genotyping and primer details


Genotyping and primer details for 787F01 - At3g06860
Line ID 787F01
Locus At3g06860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2165100 of line 787F01 Chr3:2165100
   
Insertion allele amplicon 8474 - 33ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 33ve: 5'-GAGTGGTTTTACTCACTCCACACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2164603
   
wt amplicon (over InsPos) 77vs - 33ve
wt amplicon start Chr3:2164603
wt amplicon end Chr3:2165807
wt amplicon size 1205 bp
Locus specific primer 33ve: 5'-GAGTGGTTTTACTCACTCCACACA-3'
2nd locus specific primer 77vs: 5'-TACGAACTCACCAAAAGAACTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11