Sequences from confirmation amplicon


Confirmation sequences for line 797G06 and hit At5g04140 are shown.
Line ID 797G06
Locus At5g04140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1135356
Primers used for PCR AC17 - 8474, show primer details
   
Sequence Name 39-K027919-022-797-G06C-8409
GenBank Accession LN503234 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K027919-022-797-G06C-8409
CATTTAAATTGGTAACTTTTCAATGTATTCTCTCAGGTTGCTTCAGGGCGTTTTGGAGTC
ACACCAACGTTCTTGGTCAATGCAGATCAATTGGAAATCAAAGTTGCACAAGGTGCCAAG
CCTGGGGAAGGTGGTCAGCTTCCTGGAAAGAAAGTTAGTGCGTATATCGCTAGGCTAAGA
AGCTCTAAACCTGGTGTTCCGCTTATATCTCCGCCTCCTCACCACGACATTTACTCTATT
GAGGATCTTGCTCAGTTGATCTTTGATCTACATCAGGTGATCAATCTTTTCAGAACTCAA
TAAAATGTTGAGCTAAACAAAAGGACTAACTTCCAACTTAACAAACAAACAGATTAATCC
AAATGCAAAAGTATCAGTCAAGCTAGTTGCAGAAGCTGGAATCGGAACTGTTGCTTCAGG
AGTTGCAAAGGGTAACGCTGATATCATCCAGGTAACTTGAAAGTTGAAAATATAACATTG
CATCTTGTTACTCCCATGTTCTTGTGCCGTCTCATTTTGTGTCTGCCAAGAAGTTTCATT
TTGTTCGTTTTGATATGTTTTTC
   
Sequence Name 40-K027919-022-797-G06C-AC17
GenBank Accession LN503587 [GenBank]
Sequencing primer gene specific primer, AC17: 5'-ATCATGGCCTGATATCTGAAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K027919-022-797-G06C-AC19
CTTCTTGGCAGACACAAAANTGAGACGGCACAAGAACATGGGAGTAACAAGATGCAATGT
TATATTTTCAACTTTCAAGTTACCTGGATGATATCAGCGTTACCCTTTGCAACTCCTGAA
GCAACAGTTCCGATTCCAGCTTCTGCAACTAGCTTGACTGATACTTTTGCATTTGGATTA
ATCTGTTTGTTTGTTAAGTTGGAAGTTAGTCCTTTTGTTTAGCTCAACATTTTATTGAGT
TCTGAAAAGATTGATCACCTGATGTAGATCAAAGATCAACTGAGCAAGATCCTCAATAGA
GTAAATGTCGTGGTGAGGAGGCGGAGATATAAGCGGAACACCAGGTTTAGAGCTTCTTAG
CCTAGCGATATACGCACTAACTTTCTTTCCAGGAAGCTGACCACCTTCCCCAGGCTTGGC
ACCTTGTGCAACTTTGATTTCCAATTGATCTGCATTGACCAAGAACGTTGGTGTGACTCC
AAAACGCCCTGAAGCAACCTGAGAGAATACATTTGAAAAGTTACCAATTTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAAT

Last Updated on 06.12.2018 16:11