Genotyping and primer details


Genotyping and primer details for 797G06 - At5g04140
Line ID 797G06
Locus At5g04140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1135356 of line 797G06 Chr5:1135356
   
Insertion allele amplicon 8474 - AC17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AC17: 5'-ATCATGGCCTGATATCTGAAAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1135927
   
wt amplicon (over InsPos) 77dy - AC17
wt amplicon start Chr5:1134936
wt amplicon end Chr5:1135927
wt amplicon size 992 bp
Locus specific primer AC17: 5'-ATCATGGCCTGATATCTGAAAAAC-3'
2nd locus specific primer 77dy: 5'-GATCCCAGTAGGGAAAGTAGAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11