Sequences from confirmation amplicon


Confirmation sequences for line 798F08 and hit At5g20730 are shown.
Line ID 798F08
Locus At5g20730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7019111
Primers used for PCR 31us - 8474, show primer details
   
Sequence Name 41-K117403-0022-798-F08C-31us
GenBank Accession LN504235 [GenBank]
Sequencing primer gene specific primer, 31us: 5'-TGAACCGGTTTTAACTCCTTTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117403-0022-798-F08C-31us
AATTTTTGTACCATTTCATTTCGACACAACTTCTTTGAATCTCCTCAAGGACGGAATTTC
CGGTGCTCTTGCAGATGATGAGACTGACATGGAGTCTGCACTGAAGAGAGCAATGCCATG
GCTTGATAATAGCTTAGAGATGAAAGACCCTTCGAGTACTATCTTTCCTGGTCTGAGTTT
AGTTCAGTGGATGACTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 17-K117402-0022-798-F08C-8409
GenBank Accession LN494036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117402-0022-798-F08C-8409
ACTAAACTCAGACCAGGAAAGATAGTACTCGAAGGGTCTTTCATCTCTAAGCTATTATCA
AGCCATGGCATTGCTCTCTTCAGTGCAGACTCCATGTCAGTCTCATCATCTGCAAGAGCA
CCGGAAATTCCGTCCTTGAGGAGATTCAAAGAAGTTGTGTCGAAATGAAATGGTACAAAA
ATTTACCTGGCATTCCAGGTTGTCCAGAAAAGCGAGGTCGGAAAAATGGAGGAGGACATA
TGTAGAAAGGAGTTAAACCCGGTTC

Last Updated on 06.12.2018 16:11