Genotyping and primer details


Genotyping and primer details for 798F08 - At5g20730
Line ID 798F08
Locus At5g20730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7019111 of line 798F08 Chr5:7019111
   
Insertion allele amplicon 8474 - 31us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31us: 5'-TGAACCGGTTTTAACTCCTTTCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7019376
   
wt amplicon (over InsPos) 56vd - 31us
wt amplicon start Chr5:7018508
wt amplicon end Chr5:7019376
wt amplicon size 869 bp
Locus specific primer 31us: 5'-TGAACCGGTTTTAACTCCTTTCTA-3'
2nd locus specific primer 56vd: 5'-TGTTGACCATTGTAAAGCTGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11