Sequences from confirmation amplicon


Confirmation sequences for line 817H01 and hit At3g55620 are shown.
Line ID 817H01
Locus At3g55620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20635013
Primers used for PCR KU03 - 8474, show primer details
   
Sequence Name 61-K102176-0022-817-H01C-8409
GenBank Accession LN510477 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K102176-0022-817-H01C-8409
GCCATTTACATTGAAATAATCACGATGATTTGAAGCAAGAAAATATAACAGCTAGTTGAG
AAAGCTAACCAGCACAAAGACGCCCAATAATTCGTGTTCCTCCAATAGAGGTCTTAACAA
TAGGGATAACATCTGCTAACTCCGACTCAAAAGCACTACACAAATCATCAAACCAAAAAC
ATCAAAAAAGTGAAATAGAAGAAGCAAAAACAATACTGAAAACTTTACAGAGAAAAAAAA
AAAAGGTACCTGTAAAAGTTCTCAAAGCCTCCTATAGCGACTAAGCAATAGGCATTAGTG
AGTTTGGAAAAAACACCAACTTCCCAGTTGTTCTCAAACTGAAAACCTGCGTATTACCCA
CAAATCCCATTAAGGTCAAATACCCAGAAAAGTAAACCCAACAATATCATAAGTAAGCTC
TGCAAAAAACTTACAAGTCCCCTTTAGAGGGAGGAGGCTAACGTACACCTGCGTAAATAA
AGAAACATGAAAACGAAATTAGGGTTTATATACTTCACAAATATCAACAATTCGTTAACA
ATTAGGGTTCACAAAATCCATGTCTCTAAAACAAAAAGTTTCAATTTTTCAAT
   
Sequence Name 61-K102180-0022-817-H01C-KU03
GenBank Accession LN510478 [GenBank]
Sequencing primer gene specific primer, KU03: 5'-CCCCGTCTTGTAATATAATCGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K102180-0022-817-H01C-KU03
GATTTTGTGACCCTAATTGTTACGAATCGTTGATATCTGTGACGTATATAAACCCTAATT
TCGTTTTCATGTTTCTTTATCTACGCAGGTGTACGTTAGCCTCCTCCCTCTAATGGCGAC
TCGTAAGTTTTCTGCAGAGCTTACTTATGATATTGTTGTGTTTACTCTTCTGTGTATTTG
ACCTTAATGGGATTTGTGGGTAATACGCAGGTCTTCAGTTTGAGAACAACTGCGAAGTTG
GTGTCTTTTCCAAACTCACTAATGCCTATTGCTTAGTCGCTATAGGAGGCTCTGAGAACT
TTTACAGGTACCTTTTTTTTTTTTCTCTGTAAAGTTTTCAGTATTGTTTTTGCTTCTTCT
ATTTCACTTTTTTGATGTTTTTGGTTTGATGATTTGTGTAGGGCTTTTGAGTCGAATTTA
GCAAATGTTATCCCTATTGTTAAAACCTCTTTGGGAGGAACACAAATTATTGGGCGTCTT
TGTGCTGGTTAGCTTTCTCAACTAGCTGTTATATTTTCTTGCTTCAAATCATCGGGATTA
TTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATG
GTCAATATGGAGAAAAAAAAAGAGTATTTCCCATTTTTTTTTCAATTCAAAAATGTAAAT
TTC

Last Updated on 06.12.2018 16:11