Genotyping and primer details


Genotyping and primer details for 817H01 - At3g55620
Line ID 817H01
Locus At3g55620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20635013 of line 817H01 Chr3:20635013
   
Insertion allele amplicon 8474 - KU03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU03: 5'-CCCCGTCTTGTAATATAATCGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20634415
   
wt amplicon (over InsPos) 01dt - KU03
wt amplicon start Chr3:20634415
wt amplicon end Chr3:20635280
wt amplicon size 866 bp
Locus specific primer KU03: 5'-CCCCGTCTTGTAATATAATCGAAA-3'
2nd locus specific primer 01dt: 5'-AAACTGAAATGAAACAGGTATGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11