Sequences from confirmation amplicon


Confirmation sequences for line 840C06 and hit At1g71710 are shown.
Line ID 840C06
Locus At1g71710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26974416
Primers used for PCR 65gp - 8474, show primer details
   
Sequence Name 31-K112479-0022-840-C06C-65gp
GenBank Accession LN500227 [GenBank]
Sequencing primer gene specific primer, 65gp: 5'-GATCTGAGAATTTCTGTTCGGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112479-0022-840-C06C-65gp
AGATAGTACTCTGTCACACCAAGCTGGTGTTCTTTTCGGCGCCTCTCCCTTCATTTGCGG
TGTACTCATCGGAATTCGCTTGGTACTTATAAGTTGGTGGAAAATGTAGAGTTCCTTCTG
ACCATCCATCAAATGCTCTACCTTTTCTGTATTCCTTTACCAGCTGCATTATATAATAAT
CATAAGTAACAAAATGTGTCACAACATAAGTTGAATAAACTTCAGAGCTATGGTCGGATT
TGAGACAGTGTGTGTGTATTTTTATTTGCATTAGACGTCTTCTAAAGTTATATTTGAGAT
CCAAATCCTAACAGTAACAAAGGTAATCCAAATAATTACCTGGTCATATTCAAGTAATTT
AGACCATTCTCTCTTGGAAATGAGGTCTCTGGTTTTCTCATACGATGAACTAAGCCGATA
GTTGAGATCACCTAGCCNGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 01-K112478-0022-840-C06C-8409
GenBank Accession LN486639 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112478-0022-840-C06C-8409
CATTTCATTGAATATATCCTGTCCTGGCTAGGTGATCTCAACTATCGGCTTATTTCATCG
TATGAGAAAACCAGAGACCTCATTTCCAAGAGAGAATGGTCTAAATTACTTGAATATGAC
CAGGTAATTATTTGGATTACCTTTGTTACTGTTAGGATTTGGATCTCAAATATAACTTTA
GAAGACGTCTAATGCAAATAAAAATACACACACACTGTCTCAAATCCGACCATAGCTCTG
AAGTTTATTCAACTTATGTTGTGACACATTTTGTTACTTATGATTATTATATAATGCAGC
TGGTAAAGGAATACAGAAAAGGTAGAGCATTTGATGGATGGTCAGAAGGAACTCTACATT
TTCCACCAACTTATAAGTACCAAGCGAATTCCGATGAGTACACCGCANACGATGGAAAGG
CGCCGAAAAGAACACCAGCTTGGTGTGACAGAGTACTATCCTACGGGAAAGGAATGAGGT
TGGTTCATTACAGGAGAACCGAACAGAAATTCTCAGAT

Last Updated on 06.12.2018 16:11