Genotyping and primer details


Genotyping and primer details for 840C06 - At1g71710
Line ID 840C06
Locus At1g71710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26974416 of line 840C06 Chr1:26974416
   
Insertion allele amplicon 8474 - 65gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 65gp: 5'-GATCTGAGAATTTCTGTTCGGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26973921
   
wt amplicon (over InsPos) 01ig - 65gp
wt amplicon start Chr1:26973921
wt amplicon end Chr1:26974747
wt amplicon size 827 bp
Locus specific primer 65gp: 5'-GATCTGAGAATTTCTGTTCGGTTC-3'
2nd locus specific primer 01ig: 5'-TTTCAGGATTGTGAAGTGATTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11