Sequences from confirmation amplicon


Confirmation sequences for line 860G07 and hit At3g57040 are shown.
Line ID 860G07
Locus At3g57040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21110519
Primers used for PCR 19um - 8474, show primer details
   
Sequence Name 13-K117142-0022-860-G07C-19um
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 19um: 5'-ATCCCGGAGATAGGAAAGAAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117142-0022-860-G07C-19um
CCGGGGTTCCCCAATTGAAAAAAAAACCTCCTTAATTGGTAACCCCCAGTTTCCGCCAGG
GGGAAGGGCACCAAAACCCCATTTCCGGGTTTAACCGGTGGAGAAAGTTTATTTGAACCG
AAACCTTTTGGAAAAATGGCTCCAAAATCCTCCGGGCCAGGGAAAAAAGGGGGGTACCAA
AAAAAAATCCCCACCCTTGGTTGAAAAGGGGCCAAAAAAAAACCAAACCTTTTTCCGGGG
GGTTGGGAATTAAAAGAACCACCGGTGGTTCCGGGCCCAAGGGCTTGGAATTTCCGGGGT
TAAAAACAAGTTCCGGATGTAACCACCCCAAGGGCTTTTCCAAGGGCCCCGGTAAACCCC
CGGGTTGCCCCTTTAATTTTGGAAAACCCCTTTTCCCCCGGTTTTGGTGGTGGTGGTGGT
GGTGGTAATAAAATTGGTGGGGTTAAATGGGTGCCTTCCACCGGGTGTTTAAGGGAATCC
TAAAATTTCCAAATATTGGTAGCCCGGGCAGACCGGTTAATAACGGGAAATTTCCATTGG
AAAGGGGCTCCTGGCCCGGAAATCCTCCGGGGACCCCCATGGATTAGAAGGGCCAATTGG
GAAAAAAAAAAAAATAATTTCCCATTTTTTTTCCATTCCAAAAAGGAAAATAACCCC
   
Sequence Name 03-K117141-0022-860-G07C-8409
GenBank Accession LN487654 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117141-0022-860-G07C-8409
TCCTGTCAAACCAGTCATGCCAGGCATACAATAATCTGTAATTATAAGATTCACTTCAAC
TACCTGTTGAAAGCAACCAATCAAACACAACAACTTCAATAACAACAACAACAACAACAA
CAAAAACAGAAGAAAAAGTGATTTACAAAAATAAAAGAGCAAACCTGGTGATTAACAGGA
GCCTTAGAAAACGCATTTGGGTCGTTACTATCAGTACTTTGTCTTAAACCCAGAAACTCC
AAAGCCTTTGAGCCAGAATCAACAGTTGTTACTATAAATACCAAACACACAGAAAAAAGC
TTAGATCTTTCTTCGACCATTATCAAACAAAGATTGAGACTTTTTTCTTGTAACACACAT
TTATACCTTGACACGAAGACTTTTGAAGCAATCTCTCAATAAGTTTCCGATCAAATAAAC
TATCATCAACAGCTAAAACATGAAACTGCGATTCTGCTGCCATACCCATTGCAGAAACTT
GAAGATAACAAATAAAGAAGATCTTATCTCAAATCGATGAACCCAGATGTAAAGTCTTCG
ACTTTTTTTTTTTGTTCCTCCACAAAAAAAAACAATTTATTTTTTTCCCA

Last Updated on 06.12.2018 16:11