Genotyping and primer details


Genotyping and primer details for 860G07 - At3g57040
Line ID 860G07
Locus At3g57040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21110519 of line 860G07 Chr3:21110519
   
Insertion allele amplicon 8474 - 19um
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19um: 5'-ATCCCGGAGATAGGAAAGAAAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21109927
   
wt amplicon (over InsPos) 20vb - 19um
wt amplicon start Chr3:21109927
wt amplicon end Chr3:21110986
wt amplicon size 1060 bp
Locus specific primer 19um: 5'-ATCCCGGAGATAGGAAAGAAAATA-3'
2nd locus specific primer 20vb: 5'-TCTGGTTCTTCAACTTTGTTTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11