Sequences from confirmation amplicon


Confirmation sequences for line 868E09 and hit At2g40940 are shown.
Line ID 868E09
Locus At2g40940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17085423
Primers used for PCR KQ65 - 8474, show primer details
   
Sequence Name 24-K101500-0022-868-E09C-8409
GenBank Accession LN496932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K101500-0022-868-E09C-8409
GCCATTTACATTGAATATGGGCATATTGGATCTTTGAAGTCCATTATATATGTCTTACAT
GAATAACATGGGCGCTTTGAATCTGCAGGTCATCTCTTTGATAAAGCCAATCGCATCAGT
GAAGAAACTATCAACGAATCTGATTCTGTCTGCAGACTTACCAACTTATGCTATTGGTGA
TGAGAAACGTCTGATGCAAACAATTCTTAACATCATGGGCAACGCTGTGAAATTTACTAA
GGAAGGCTACATCTCCATAATAGCCTCTATCATGAAACCCGAGTCCTTACAAGAATTACC
ATCTCCAGAATTTTTTCCAGTTCTCAGTGACAGTCACTTCTACCTATGTGTGCAGGTTAG
ACCCAATCTACAAATTACTAAACTACAAAGTTAAGCTTCTTACTGTGTTCTTACTGTTAT
AATCATGGTGCAGGTGAAGGACACAGGGTGTGGAATTCACACACAAGACATTCCTTTGCT
CTTT
   
Sequence Name 24-K101504-0022-868-E09C-KQ65
GenBank Accession LN496933 [GenBank]
Sequencing primer gene specific primer, KQ65: 5'-GGTAAAGAGCAAAGGAATGTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K101504-0022-868-E09C-KQ65
ATTATACAGTAAGAACACAGTAAGAAGCTTAACTTTGTAGTTTAGTAATTTGTAGATTGG
GTCTAACCTGCACACATAGGTAGAAGTGACTGTCACTGAGAACTGGAAAAAATTCTGGAG
ATGGTAATTCTTGTAAGGACTCGGGTTTCATGATAGAGGCTATTATGGAGATGTAGCCTT
CCTTAGTAAATTTCACAGCGTTGCCCATGATGTTAAGAATTGTTTGCATCAGACGTTTCT
CATCACCAATAGCATAAGTTGGTAAGTCTGCAGACAGAATCAGATTCGTTGATAGTTTCT
TCACTGATGCGATTGGCTTTATCAAAGAGATGACCTGCAGATTCAAAGCGCCCATGTTAT
TCATGTAAGACATATATNATGGACTTCAAAGATCCCATATGCCCATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCG

Last Updated on 06.12.2018 16:11