Genotyping and primer details


Genotyping and primer details for 868E09 - At2g40940
Line ID 868E09
Locus At2g40940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17085423 of line 868E09 Chr2:17085423
   
Insertion allele amplicon 8474 - KQ65
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KQ65: 5'-GGTAAAGAGCAAAGGAATGTCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17084971
   
wt amplicon (over InsPos) 12bj - KQ65
wt amplicon start Chr2:17084971
wt amplicon end Chr2:17086051
wt amplicon size 1081 bp
Locus specific primer KQ65: 5'-GGTAAAGAGCAAAGGAATGTCTTG-3'
2nd locus specific primer 12bj: 5'-CTCTCTAATTTCCAAGGCAGTGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11